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l 美國Hausser scientific血球計數板,準確計算白細胞、紅細胞、血小板、組織培養細胞、細菌和精子
l H形兩個計數區,V型斜槽防止液體溢出
l 气村When viewed through a microscope, hemacytometers display white Neubauer markings against a dark background. Choose from Bright-Line® hemacytometers with a Pyrex® surface or Hy-lite hemacytometers with a milled glass surface. Bright-Line phase contrast hemacytometer is thinner—ideal for use with phase contrast microscopes. Glass coverslips cover moat region 0.1 mm above ruled surface, limiting volume to 0.1 mm3
貨號 | 產品描述 |
3100 | 美國Hausser scientific血球計數板 |
| HEMACYTOMETER HY-LITE血球計數板 |
| HEMACYTOMETER PHASE CONTRAST血球計數板 |
| HEMACYTOMETER蓋玻片 |
3110 | Hemacytometer hy-lite血球計數板, counting chamber |
裆原The Bright-Line Hemacytometer is of one piece construction, ensuring long term durability and accuracy. An H-shaped moat forms two counting areas, or plateaus. A "V" slash at the loading side of each plateau facilitates charing and reduces the possibility of overflow into the moat. Each plateau features enhanced Neubauer rulings. The ruled surface is 0.1mm below the cover glass, limiting the volume of blood or fluid over a square mm at 0.1cu.mm and over each of 400 squares (within the central square mm) to 0.00025 cu. mm. Contact of the flat, polished cover glass surfaces with cover glass supports produces an exact volume of fluid over the counting area. The difference in surface tension characteristics between the metallic surface on the chamber and the polished cover glass assures smooth capillarity for precise loading and more even cell distribution.
Directions for Use
租桑Bright-Line / Dark-Line Counting Chambers Catalog Numbers: 3100, 3110, 3200, 3500, 1490, 1492, 1475, & 1483
Usage: Cell Counts
Cell Depth: 0.100mm +/- 2% (1/10mm)
Volume: 0.1 Microliter
封孙Ruling Pattern: Improved Neubauer, 1/400 Square mm
劝贸Rulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. (These are indicated in the illustration below.) The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10mm below the cover glass, so that the volume over each of the 16 small squares is .00025 cubic mm.
The number of cells per cubic millimeter =
尔店 Number of cells counted per square millimeter X dilution (if used) X 10
The number of cells per milliliter =
屏勾 Number of cells counted per square millimeter X dilution (if used) X 10,000
赢苟One (1) Milliliter = 1000 cubic millimeters (cu mm)
喂熄One (1) Microliter (ul) = One (1) cubic millimeter (cu mm)
害碾To clean the counting chamber: After completing the count, remove the cover glass and clean the counting chamber with water or a mild cleaning solution (10% solution of bleach). Dry the counting chamber with a soft cloth or wipe, or rinse with acetone.
=MsoNormal style='margin-left:21.0pt;text-indent:-21.0pt;mso-list:l2 level1 lfo2'>l 隙殴Each large square has a surface area of 1.0mm2, and the depth of the chamber is 0.1mm. As there are 1000 mm3 per ml, each large square represents a volume of 0.0001ml, so that it is equal to 1/ 0.0001ml = 10,000 = (104)
l 艾猜Cells per ml = Average count per square x dilution factor x 10,000
企業證書
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